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प्रश्न
What does ‘competent’ refer to in competent cells used in transformation experiments?
उत्तर
Competent means bacterial cells, on treatment with CaCl2, are made capable of taking up foreign DNA.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Why is the coding sequence of an enzyme β - galactosidase a preferred selectable marker in comparison to the ones named above?
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
Which of the following statement is not true for a clone?
A suitable vector for gene cloning in higher organism is ______.
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Which of the following is not a cloning vector?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
