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प्रश्न
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
उत्तर
Agrobacterium is a bacterium that infects plant tissues by transferring its plasmid T-DNA to the plant genome. This property of Agrobacterium is exploited in the biotechnology industry.
The desired gene that has to be to transferred to a particular plant is inserted in a plasmid T-DNA of Agrobacterium. Then, this engineered Agrobacterium is allowed to infect that particular plant, so that it can transfer the desired gene to the plant genome through its T-DNA. In this way, the desired changes are made in the plant.
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संबंधित प्रश्न
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Describe briefly the following:
Origin of replication
What is ‘Ori’ ? State its importance during cloning of a vector.
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a genetic vector?
Which of the following is not a cloning vector?
What does ‘competent’ refer to in competent cells used in transformation experiments?
