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प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
उत्तर
The naming of a restriction endonuclease is done in the following way:
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Ist letter represents the genus of the organism from which the enzyme is derived.
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IInd and IIIrd letters represent the species of the organism.
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IVth letter represents the name of the strain.
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Roman number represents order of isolation.
For example, In EcoRI − Derived from E.coli, strain R.
It is the Ist to be discovered.
संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Explain briefly:
Restriction enzymes and DNA
How does restriction endonuclease function?
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
Molecular scissors, which cut DNA at specific site is ______.
DNA fragments separate according to size through?
Which of the following statements does not hold true for restriction enzyme?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
How does one visualise DNA on an agarose gel?
