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प्रश्न
How does restriction endonuclease function?
उत्तर
Each restriction endonuclease finds its specific palindromic nucleotide sequences in the DNA and cuts the DNA at these specific sites. It does so by binding to the DNA at these sites and cutting both the strands at specific points in their sugar-phosphate backbones.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Restriction enzymes ______.
'Restriction' in Restriction enzyme refers to ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
How does one visualise DNA on an agarose gel?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
State the principle involved in separation of DNA fragments using gel electrophoresis.
