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प्रश्न
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
विकल्प
Formation of phosphodiester bond between two DNA fragments
Formation of hydrogen bonds between sticky ends of DNA fragments
Ligation of all purime and pyrimidine bases
None of the above
उत्तर
The role of DNA ligase in the construction of a recombinant DNA molecule is formation of phosphodiester bond between two DNA fragments.
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संबंधित प्रश्न
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
How does a restriction nuclease function? Explain
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Give a reason why :
Single cloning site is preferred in a vector.
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
DNA fragments separate according to size through?
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
How are DNA fragments visualised once they are separated by gel electrophoresis?
