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प्रश्न
How does a restriction nuclease function? Explain
उत्तर
Restriction endonuclease functions by recognising its specific sequence. It binds to the DNA and cuts each to the two strands of the double helix at specific points in their sugar phosphate backbones. These enzymes cut the strand of DNA between the same two bases on the opposite strands leaving a single-stranded portion at their ends. These overhanging stretches are called sticky ends on each strand. The enzyme DNA ligase functions depending on the stickiness of these ends. For example, the restriction endonuclease EcoRI found in the colon bacteria E. coli recognises the base sequence GAATTC in the DNA duplex and cuts its strands between G and A.
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संबंधित प्रश्न
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Give a reason why :
Single cloning site is preferred in a vector.
'Restriction' in restriction enzyme refers to
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
