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प्रश्न
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
उत्तर
On finding the palindrome, the endonuclease binds to the DNA.
- It cuts the opposite strands of DNA, but between the same bases on both the strands & forms STICKY ENDS. This sticky ends facilitate the action of enzyme DNA ligase and help in the formation of recombination DNA.
APPEARS IN
संबंधित प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
Which of the following radioisotope is not suitable for DNA labeling based studies?
DNA fragments separate according to size through?
Which of the following bacteria is not a source of restriction endonuclease?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
State the importance of elution in this process.
