Advertisements
Advertisements
प्रश्न
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
उत्तर
Role of Heat: It helps in the denaturation process in PCR. The ds DNA is heated in this process at a very high temperature (95ºC) so that both the strands separates.
Role of primers: Primers are chemically synthesised small oligonucleotides of about 10-18 nucleotides that are complementary to a region of template DNA and helps in the extension of the new chain.
Role of Bacterium Thermus aquaticus: From this bacterium, a thermostable Taq DNA polymerase is isolated which can tolerate high temperatures and forms a new strand.
APPEARS IN
संबंधित प्रश्न
Consult internet and find out how to make orally active protein pharmaceutical. What is the major problem to be encountered?
Give a schematic representation of the transformation of a pro-insulin into insulin.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
ELISA is mainly used for ____________.
Which of the following is a neuropeptide hormone?
Choose the correct option regarding retrovirus.
Which is false about antibiotics?
What is a recombinant DNA vaccine? Give two examples.
Who was the first patient who was given gene therapy? Why was the given treatment recurrent in nature?
How does a gene therapy involving direct modification of the cells, in order to achieve a therapeutic goal is used in the treatment of ADA deficiency? Explain.
