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प्रश्न
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
उत्तर
Role of Heat: It helps in the denaturation process in PCR. The ds DNA is heated in this process at a very high temperature (95ºC) so that both the strands separates.
Role of primers: Primers are chemically synthesised small oligonucleotides of about 10-18 nucleotides that are complementary to a region of template DNA and helps in the extension of the new chain.
Role of Bacterium Thermus aquaticus: From this bacterium, a thermostable Taq DNA polymerase is isolated which can tolerate high temperatures and forms a new strand.
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संबंधित प्रश्न
Expand the Given and mention one application of each:
PCR
Suggest any two possible treatments that can be given to a patient exhibiting adenosine deaminase deficiency.
State the role of C peptide in human insulin.
Describe the role of primers.
Describe the role of bacterium Thermus aquaticus in carrying the process of polymerase chain reaction.
PCR proceeds in three distinct steps governed by temperature, they are in order of ____________.
PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
What is a recombinant DNA vaccine? Give two examples.
Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
A cell-free method of amplifying DNA first developed in the mid 1980's revolutionised the field of biotechnology, Name the method and explain the basic steps of the technique involved.
