Advertisements
Advertisements
Question
Answer the following question.
Describe the roles of heat, primers, and the bacterium Thermus aquaticus in the process of PCR.
Solution
Role of Heat: It helps in the denaturation process in PCR. The ds DNA is heated in this process at a very high temperature (95ºC) so that both the strands separates.
Role of primers: Primers are chemically synthesised small oligonucleotides of about 10-18 nucleotides that are complementary to a region of template DNA and helps in the extension of the new chain.
Role of Bacterium Thermus aquaticus: From this bacterium, a thermostable Taq DNA polymerase is isolated which can tolerate high temperatures and forms a new strand.
APPEARS IN
RELATED QUESTIONS
Diagrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
What is gene therapy? Name the first clinical case in which it was used.
Answer the following question.
Explain the various steps involved in the production of artificial insulin.
Explain how ADA deficiency can be corrected?
ELISA is based on:
Which of the following is the correct sequence of steps in a PCR (Polymerase Chain Reaction)?
A patient is suffering from ADA deficiency. Can he be cured? How?
A host cell must be made competent, before it is able to receive an rDNA. Justify.
In humans, somatic gene therapy was carried out to correct an immunodeficiency disease. Name this disease.
Give a reason for the following:
Streptokinase is administered to the patients having myocardial infarction.
