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प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
उत्तर
Exonuclease removes the nucleotides from the ends of the DNA chain while endonuclease cuts the DNA at the specific positions within the DNA strand.
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
How does a restriction nuclease function? Explain
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
State the importance of elution in this process.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
