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प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
उत्तर
Exonuclease removes the nucleotides from the ends of the DNA chain while endonuclease cuts the DNA at the specific positions within the DNA strand.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Do eukaryotic cells have restriction endonucleases? Justify your answer.
'Restriction' in restriction enzyme refers to
Which of the following statements does not hold true for restriction enzyme?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
What is elution?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
How are DNA fragments visualised once they are separated by gel electrophoresis?
