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प्रश्न
What is elution?
उत्तर
The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution.
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संबंधित प्रश्न
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Give a reason why :
Single cloning site is preferred in a vector.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
'Restriction' in Restriction enzyme refers to ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
