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प्रश्न
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
उत्तर
If the restriction enzymes have more than one recognition site in a vector then the vector itself will get fragmented on treatment with the restriction enzyme.
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संबंधित प्रश्न
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Distinguish between exonuclease and endonuclease
How does restriction endonuclease function?
Give a reason why :
Single cloning site is preferred in a vector.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
DNA fragments separate according to size through?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
What is elution?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
