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प्रश्न
Distinguish between exonuclease and endonuclease
उत्तर
| Sr. No. | Exonuclease | Endonuclease |
|
1. |
It is a type of restriction enzyme that removes the nucleotide from the 5’ or 3’ ends of the DNA molecule. |
It is a type of restriction enzyme that makes a cut within the DNA at a specific site to generate sticky ends. |
|
2. |
They separated DNA base pairs at their terminal ends.
|
They separate DNA at any location other than the terminal ends.
|
| 3. | They work on single strands of DNA or gaps in double-stranded DNA. | They cut one or both strands of double-stranded DNA. |
APPEARS IN
संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Mention the difference in the mode of action of exonuclease and endonuclease.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
Molecular scissors, which cut DNA at specific site is ______.
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
While isolating DNA from bacteria, which of the following enzymes is not required?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Which of the following bacteria is not a source of restriction endonuclease?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
State the principle involved in separation of DNA fragments using gel electrophoresis.
How are DNA fragments visualised once they are separated by gel electrophoresis?
Identify the activity of endonuclease and exonuclease in the given image.
