English
Karnataka Board PUCPUC Science 2nd PUC Class 12

Distinguish between exonuclease and endonuclease - Biology

Advertisements
Advertisements

Question

Distinguish between exonuclease and endonuclease

Distinguish Between

Solution

Sr. No. Exonuclease Endonuclease

1.

It is a type of restriction enzyme that removes the nucleotide from the 5’ or 3’ ends of the DNA molecule.

It is a type of restriction enzyme that makes a cut within the DNA at a specific site to generate sticky ends.

2.

They separated DNA base pairs at their terminal ends.

They separate DNA at any location other than the terminal ends.

3. They work on single strands of DNA or gaps in double-stranded DNA. They cut one or both strands of double-stranded DNA.
shaalaa.com
Tools of Recombinant DNA Technology - Restriction Enzymes
  Is there an error in this question or solution?
Chapter 11: Biotechnology : Principles and Processes - Exercises [Page 206]

APPEARS IN

NCERT Biology [English] Class 12
Chapter 11 Biotechnology : Principles and Processes
Exercises | Q 12.3 | Page 206

RELATED QUESTIONS

How are 'sticky ends' formed on a DNA strand? Why are they so called? 


Mention the difference in the mode of action of exonuclease and endonuclease.


Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease


Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.


Explain briefly:

Restriction enzymes and DNA


Explain the roles of the following with the help of an example each in recombinant DNA technology :

Restriction Enzymes


The DNA fragment separated on an agarose gel can be visualized by staining with ______.


Which of the following radioisotope is not suitable for DNA labeling based studies?


There is a restriction endonudease called as EcoRI. What does co part in it stands for?


Molecular scissors, which cut DNA at specific site is ______.


Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?


'Restriction' in Restriction enzyme refers to ______.


While isolating DNA from bacteria, which of the following enzymes is not required?


Which of the following bacteria is not a source of restriction endonuclease?


What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?


Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.


Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?


How does one visualise DNA on an agarose gel?


Identify the activity of endonuclease and exonuclease in the given image.


Share
Notifications

Englishहिंदीमराठी


      Forgot password?
Use app×