Advertisements
Advertisements
Question
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
Options
DNA can be seen in visible light
DNA can be seen without staining in visible light
Ethidium bromide stained DNA can be seen in visible light
Ethidium bromide stained DNA can be seen under exposure to UV light
Solution
Ethidium bromide stained DNA can be seen under exposure to UV light
APPEARS IN
RELATED QUESTIONS
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Give a reason why :
Single cloning site is preferred in a vector.
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Would you choose an exonuclease while producing a recombinant DNA molecule?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
Identify the activity of endonuclease and exonuclease in the given image.
