Advertisements
Advertisements
Question
How does one visualise DNA on an agarose gel?
Solution
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultraviolet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium Bromide and UV.
APPEARS IN
RELATED QUESTIONS
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Give a reason why :
Single cloning site is preferred in a vector.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
'Restriction' in Restriction enzyme refers to ______.
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
State the importance of elution in this process.