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प्रश्न
How does one visualise DNA on an agarose gel?
उत्तर
A compound called Ethidium Bromide stains DNA, which on irradiating with Ultraviolet, fluoresce gives orange light. Hence, DNA fragments appear as orange band in the presence of Ethidium Bromide and UV.
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संबंधित प्रश्न
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Distinguish between exonuclease and endonuclease
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
While isolating DNA from bacteria, which of the following enzymes is not required?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
