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प्रश्न
Do eukaryotic cells have restriction endonucleases? Justify your answer.
उत्तर
Yes, restriction endonucleases are found in eukaryotic cells. Restriction endonuclease acts by examining the length of the DNA sequence. When it finds its specific recognition sequence, it binds to the DNA and cuts both strands of the double helix at specific points in the sugar-phosphate backbones. Each restriction endonuclease recognises specific palindromic nucleotide sequences in the DNA.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
How does a restriction nuclease function? Explain
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Explain briefly:
Restriction enzymes and DNA
Distinguish between exonuclease and endonuclease
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Which of the following radioisotope is not suitable for DNA labeling based studies?
Molecular scissors, which cut DNA at specific site is ______.
DNA fragments separate according to size through?
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
Which of the following bacteria is not a source of restriction endonuclease?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
State the principle involved in separation of DNA fragments using gel electrophoresis.
