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Question
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Solution
Yes, restriction endonucleases are found in eukaryotic cells. Restriction endonuclease acts by examining the length of the DNA sequence. When it finds its specific recognition sequence, it binds to the DNA and cuts both strands of the double helix at specific points in the sugar-phosphate backbones. Each restriction endonuclease recognises specific palindromic nucleotide sequences in the DNA.
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RELATED QUESTIONS
Explain with the help of a suitable example the naming of a restriction endonuclease.
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CTTAAG
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- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
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(b) The above setup was modified and a band with 250 base pairs was obtained at X.
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