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प्रश्न
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
उत्तर
- Palindromic sequences, endonuclease enzyme
- Restriction enzymes can make complementary cut counterparts forming sticky ends for recombination DNA/RDNA technology to facilitate the ligation of vector and foreign DNA.
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Give a reason why :
Single cloning site is preferred in a vector.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
'Restriction' in Restriction enzyme refers to ______.
Which of the following bacteria is not a source of restriction endonuclease?
Which of the following statements does not hold true for restriction enzyme?
How does one visualise DNA on an agarose gel?
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
What is elution?
