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प्रश्न
State the principle involved in separation of DNA fragments using gel electrophoresis.
उत्तर
As negatively charged molecules, DNA fragments can be forced to migrate in the direction of the anode through a medium or matrix in order to be separated. Through the sifting effect that the agarose gel provides, the DNA fragments separate (resolve) according to their size. Therefore, the size of the fragment determines how far it travels.
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संबंधित प्रश्न
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Would you choose an exonuclease while producing a recombinant DNA molecule?
How does one visualise DNA on an agarose gel?
What is elution?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
How are DNA fragments visualised once they are separated by gel electrophoresis?
Identify the activity of endonuclease and exonuclease in the given image.
