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प्रश्न
State the principle involved in separation of DNA fragments using gel electrophoresis.
उत्तर
As negatively charged molecules, DNA fragments can be forced to migrate in the direction of the anode through a medium or matrix in order to be separated. Through the sifting effect that the agarose gel provides, the DNA fragments separate (resolve) according to their size. Therefore, the size of the fragment determines how far it travels.
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Do eukaryotic cells have restriction endonucleases? Justify your answer.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
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How does one visualise DNA on an agarose gel?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
