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प्रश्न
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
पर्याय
Formation of phosphodiester bond between two DNA fragments
Formation of hydrogen bonds between sticky ends of DNA fragments
Ligation of all purime and pyrimidine bases
None of the above
उत्तर
The role of DNA ligase in the construction of a recombinant DNA molecule is formation of phosphodiester bond between two DNA fragments.
APPEARS IN
संबंधित प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
How does restriction endonuclease function?
Molecular scissors, which cut DNA at specific site is ______.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
How does one visualise DNA on an agarose gel?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
State the principle involved in separation of DNA fragments using gel electrophoresis.
How are DNA fragments visualised once they are separated by gel electrophoresis?
