Advertisements
Advertisements
Question
State the importance of elution in this process.
Solution
The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.
APPEARS IN
RELATED QUESTIONS
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
Which of the following radioisotope is not suitable for DNA labeling based studies?
'Restriction' in Restriction enzyme refers to ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
What is elution?
