Advertisements
Advertisements
Question
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
Solution
- Anode = S
- Smallest/lightest DNA Strand = R
- Agarose gel = T
APPEARS IN
RELATED QUESTIONS
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Distinguish between exonuclease and endonuclease
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Restriction enzymes ______.
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
While isolating DNA from bacteria, which of the following enzymes is not required?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
State the principle involved in separation of DNA fragments using gel electrophoresis.
