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प्रश्न
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
उत्तर
'YAC' → Yeast Artificial Chromosome :
It is used as a cloning vector for cloning DNA fragments in the suitable host so that DNA sequencing can be done.
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संबंधित प्रश्न
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
What is ‘Ori’ ? State its importance during cloning of a vector.
Explain the importance of ‘Selectable marker’, with the help of a suitable example.
Which of the following statement is not true for a clone?
A suitable vector for gene cloning in higher organism is ______.
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
What does ‘competent’ refer to in competent cells used in transformation experiments?
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.
