Advertisements
Advertisements
Question
Answer the following question.
Expand ‘YAC’ and mention what it was used for.
Solution
'YAC' → Yeast Artificial Chromosome :
It is used as a cloning vector for cloning DNA fragments in the suitable host so that DNA sequencing can be done.
APPEARS IN
RELATED QUESTIONS
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
State how has Agrobacterium tumifaciens been made a useful cloning vector to transfer DNA to plant cells.
Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
Which of the following is correctly matched?
A suitable vector for gene cloning in higher organism is ______.
Plasmids are suitable vectors for gene cloning because these ______.
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
The figure below shows the structure of a plasmid.
A foreign DNA was ligated at BamH1. The transformants were then grown in a medium containing antibiotics tetracycline and ampicillin.
Choose the correct observation for the growth of bacterial colonies from the given table.