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Question
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
Solution
In the cloning vector pBR322, ampicillin and tetracycline resistance genes serve as selectable markers. They help in the selection of transformed cells from nontransformed cells. They also help distinguish recombinant cells from nonrecombinant cells.
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RELATED QUESTIONS
Draw a schematic sketch of pBR 322 plasmid and label the following in it:
(a) Any two restriction sites.
(b) Ori and rop genes.
(c) An antibiotic resistant gene.
Can you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
Why is the coding sequence of an enzyme β - galactosidase a preferred selectable marker in comparison to the ones named above?
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
How does the β-galactosidase coding sequence act as a selectable marker? Why is it a preferred selectable marker to antibiotic resistance genes? Explain.
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of ______.
A clone is a ______.
Plasmids are suitable vectors for gene cloning because these ______.
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Which of the following is not a cloning vector?
