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प्रश्न
Name the selectable markers in the cloning vector pBR322? Mention the role they play.
उत्तर
In the cloning vector pBR322, ampicillin and tetracycline resistance genes serve as selectable markers. They help in the selection of transformed cells from nontransformed cells. They also help distinguish recombinant cells from nonrecombinant cells.
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संबंधित प्रश्न
Describe briefly the following:
Origin of replication
Write the role of Ori and 'restriction' site in a cloning vector pBR322.
Answer the following question.
β galactosidase enzyme is considered a better selectable marker. Justify the statement.
A suitable vector for gene cloning in higher organism is ______.
Plasmids are suitable vectors for gene cloning because these ______.
Which of the following is not a genetic vector?
A cloning vector should possess which of the following characters?
- Origin of replication (Ori)
- Ability to destroy the alien DNA
- Cloning site to link the alien DNA
- The tumour inducing plasmid Ti.
- Selectable marker.
- Low molecular weight
The CORRECT combination is
Which of the following is not a cloning vector?
What does ‘competent’ refer to in competent cells used in transformation experiments?
For selection of recombinants, insertional inactivation of antibiotic marker has been superceded by insertional inactivation of a marker gene coding for a chromogenic substrate. Give reasons.
