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Question
What modification is done on the Ti plasmid of Agrobacterium tumefaciens to convert it into a cloning vector?
Solution
T-DNA is the only essential part required to make Ti plasmid a cloning vector. The plasmid is disarmed by deleting the tumour-inducing genes in the plasmid so that it becomes an effective cloning vector and remove it harmful effect.
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RELATED QUESTIONS
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