Advertisements
Advertisements
Question
What is meant by gene cloning?
Solution
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
APPEARS IN
RELATED QUESTIONS
Name the most commonly used bioreactor and describe its working.
The bacterium which causes a plant disease called crown gall is _______________.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
Enlist and write in brief about the different biological tools required in rDNA technology.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
Name a recombinant vaccine that is currently being used in vaccination program?
