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प्रश्न
What is meant by gene cloning?
उत्तर
Gene cloning refers to a process in which a gene of interest is ligated to a vector. The recombinant DNA thus produced is introduced into a host cell by transformation. Each cell gets one DNA molecule and when the transformed cell grows into a bacterial colony, each cell in the colony has a copy of the gene. This is precisely gene cloning.
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संबंधित प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Enlist and write in brief about the different biological tools required in rDNA technology.
The technique which involves addition or deletion of genes is ______.
____________ cuts the DNA within the specific positions.
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Who among the following was awarded the Nobel Prize for the development of PCR technique?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
______technique is used for in vitro gene cloning or gene multiplication.
Tissue plasminogen activator and urokinase are used in the treatment of ______.
David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
