Process and Principles of Biotechnology

Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?

Explain the mechanism of PCR.

Ti plasmid being used for introducing genes in plants obtained from ______.

Enlist and write in brief about the different biological tools required in rDNA technology.

Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:

1. Name the host for this cloning vector.
2. Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
3. Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
   5' --- GTACGAATCCTGA --- 3'
   3' --- CATGCTTAGGACT --- 3'

DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.

A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?

Nucleic acid segment tagged with a radioactive molecule is called ______.