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प्रश्न
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
उत्तर
Both. As biotechnology is a very wide area which deals with techniques of using a ‘natural’ organism (or its parts) as well as genetically modified organisms to produce products and processes useful for mankind. A winemaker employs a strain-of yeast to produce wine by fermentation (a natural phenomenon), while the molecular biologist has cloned gene for the antigen (that is used as a vaccine) in an organism which allows the production of the antigen in large amount.
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संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
What are plasmids?
Describe two vectors less methods of gene transfer used in rDNA technology.
What is plasmid?
Bt-cotton shows adverse effect on the population of which butterfly?
Which of the following statements is correct with respect to cellular respiration?
Which of the following viruses has the capacity to produce lysozymes?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
The PCR technique is used for ______.
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
