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प्रश्न
What is plasmid?
उत्तर
Plasmid is a circular, double stranded, self-replicating, extra-chromosomal DNA molecule.
संबंधित प्रश्न
Distinguish between RNA and DNA
The bacterium which causes a plant disease called crown gall is _______________.
EcoRl & Hind III are the examples of ______
Human insulin is produced by culturing the genetically engineered ______
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
What is polymerase chain reaction (PCR)?
Describe three steps involved in mechanism of PCR.
Write steps involved in gene amplification.
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
