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प्रश्न
Describe three steps involved in mechanism of PCR.
उत्तर
At the start of PCR, all the requirements are mixed together in 'eppendorf tube' and the following operations are performed sequentially:
- Denaturation: The reaction mixture is heated to a temperature (90–98° C) to separate two strands of desired DNA. This is called denaturation.
- Annealing: The mixture is allowed to cool (40–60° C) that permits pairing of the primer to the complementary sequences in DNA. This step is called annealing.
- Primer extension/Polymerization: The temperature (70–75° C) allows thermostable Taq DNA polymerase to use single-stranded DNA as template and adds nucleotides. This is called primer extension. It takes around two minutes duration.
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संबंधित प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Polymerase chain reaction is most useful in ______.
Match the List I with List II and List ill. Select the correct option.
| List I | List II | List ill | |||
| i. | Hind III | a. | Agarose ge | 1. | Six base pairs |
| ii. | pBR322 | b. | Agrobacterium | 2. | Selectable marke |
| iii. | T-DNA | c. | Ampicillin | 3. | Elution |
| iv. | DNA | d. | Recognition sequenc | 4. | Transgenic plant |
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
Exonuclease performs the function of ____________.
The source of taq Polymerase used in PCR is a ______.
Annealing step of PCR operates at ______ °C.
Which vector is mostly used in rDNA technology in plants?
Name the source of thermostable DNA polymerase.
