Advertisements
Advertisements
प्रश्न
Describe three steps involved in mechanism of PCR.
उत्तर
At the start of PCR, all the requirements are mixed together in 'eppendorf tube' and the following operations are performed sequentially:
- Denaturation: The reaction mixture is heated to a temperature (90–98° C) to separate two strands of desired DNA. This is called denaturation.
- Annealing: The mixture is allowed to cool (40–60° C) that permits pairing of the primer to the complementary sequences in DNA. This step is called annealing.
- Primer extension/Polymerization: The temperature (70–75° C) allows thermostable Taq DNA polymerase to use single-stranded DNA as template and adds nucleotides. This is called primer extension. It takes around two minutes duration.
APPEARS IN
संबंधित प्रश्न
What would be the molar concentration of human DNA in a human cell?
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
Give the applications of the PCR technique.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
The source of taq Polymerase used in PCR is a ______.
Which of these are most widely used in genetic engineering?
The polymerase chain reaction is a powerful technique to ______.
How gene amplification is done?
Write a short note on vectors.
