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प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
उत्तर
Each restriction endonuclease recognizes a specific palindromic base sequence in foreign DNA and cuts the sugar-phosphate backbone of both strands of the double helix at specific points. These ends are called sticky ends. The sticky ends of 2 different molecules of DNA cut by the same restriction endonuclease arc then joined by ligase to form a recombinant DNA.
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संबंधित प्रश्न
From the following which one is NOT a feature of the plasmids?
Identify the characterization techniques that are based on molecular weights of compounds.
Which of the following is the right combination of primers in a PCR to amplify the DNA product?
The polymerase chain reaction is a powerful technique to ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
What are the different types of electrophoresis?
Write a short note on vectors.
What is EcoRI?
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
