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प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
उत्तर
Each restriction endonuclease recognizes a specific palindromic base sequence in foreign DNA and cuts the sugar-phosphate backbone of both strands of the double helix at specific points. These ends are called sticky ends. The sticky ends of 2 different molecules of DNA cut by the same restriction endonuclease arc then joined by ligase to form a recombinant DNA.
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संबंधित प्रश्न
How has the development of bioreactor helped in biotechnology?
Expand the following abbreviation: BAC
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
What is PCR? Explain different steps involved in it.
Identify the process by which bacteria protect their own DNA from the attack of restriction enzymes.
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
Which of the following is not required in the preparation of a recombinant DNA molecule?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
