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प्रश्न
Explain what are the desirable characteristics of an ideal cloning vector used in rDNA technology.
उत्तर
Desirable characters of an ideal cloning vector :
- Origin of replication: It is a specific sequence of nucleotides that serves as a binding site for initiator protein (Helicase) to start replication. Any foreign DNA linked with vector DNA also gets replicated along with it, hence it is important for maintaining the copy number of foreign DNA.
- Selectable markers: Cloning vectors must contain at least one selectable marker or it helps in:
– detection of transformed host cells,
– Identification and elimination of nontransformed post cells,
– Isolation and growth of transformed host cells. - Cloning sites: A cloning site is a restriction endonuclease recognition site or target site in vector DNA. It has a palindromic sequence of nitrogenous bases cut by a specific restriction endonuclease enzyme for insertion and linking of foreign DNA.
- Small size: A vector should be small in size because large molecules bind is break up during isolation and purification.
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संबंधित प्रश्न
One of the major contributions of biotechnology is to develop pest-resistant varieties of cotton plants. Explain how it has been made possible.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Which of the following is an INCORRECT match?
The source of taq Polymerase used in PCR is a ______.
DNA fingerprinting refers to ______.
Who among the following was awarded the Nobel Prize for the development of PCR technique?
Restriction enzymes were discovered by ______.
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
Explain the mechanism of PCR.
