Advertisements
Advertisements
प्रश्न
What are the restriction endonucleases? Give the rules of their nomenclature.
उत्तर
They cut DNA duplex at specific points. They are molecular scissors.
Rules: Restriction endonucleases are named by using the first letter of the name of the genus followed by the first two letters of species from which these enzymes are isólated. All three letters are written in italics.
APPEARS IN
संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
What is Palindromic sequence?
Define biotechnology? Which are the basic principles and process of biotechnology?
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Which of the following device is required for PCR?
Name a recombinant vaccine that is currently being used in vaccination program?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Expand the following acronym:
BAC
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
