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प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
उत्तर
Stanley Cohen and Herbert Boyer conducted one of the first genetic engineering experiments. They invented the technique of DNA cloning. Cohen developed a method of removing plasmids from the cell and then reinserting them in other cells. Combining this process with that of DNA splicing enabled Boyer and Cohen to recombine segments of DNA in desired configurations and insert the DNA in bacterial cells, which could then act as manufacturing plants for specific proteins. Stanley Cohen and Herbert Boyer accomplished this in 1972.
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संबंधित प्रश्न
Polymerase chain reaction is most useful in ______.
What is Palindromic sequence?
Explain types of enzymes used in biotechnology?
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
A hind of biotechnology involving manipulation of DNA is ______.
Restriction enzymes were discovered by ______.
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
How does EcoRI differ from an exonuclease?
Which amino acid is coded by the initiation codon?
Which one of the following enzymes is used to join DNA fragments?