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प्रश्न
Polymerase chain reaction is most useful in ______.
पर्याय
DNA amplification
DNA synthesis
protein synthesis
selective replication of DNA
उत्तर
Polymerase chain reaction is most useful in DNA amplification.
APPEARS IN
संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
Give an example of the source of thermostable enzyme DNA polymerase.
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
