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प्रश्न
Ti plasmid being used for introducing genes in plants obtained from ______.
पर्याय
Agrobacterium rhizogenes
Escherichia coli
Agrobacterium T20
Agrobacterium tumefaciens
उत्तर
Ti plasmid being used for introducing genes in plants obtained from Agrobacterium tumefaciens.
संबंधित प्रश्न
Distinguish between Plasmid DNA and chromosomal DNA
Write a short note on Electrophoresis.
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
From the following identify the methods of electrophoresis.
DNA fingerprinting refers to ______.
Which of the following is not required in the preparation of a recombinant DNA molecule?
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
