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प्रश्न
Distinguish between Plasmid DNA and chromosomal DNA
उत्तर
| Sr. no. | Plasmid DNA | Chromosomal DNA |
| 1. | Plasmids are circular external DNA that is self-replicating and are naturally found in bacteria and yeast. | Chromosomal DNA sequence, including recognition site, is present in the chromosomes of all organisms. |
| 2. | It is not necessary for normal growth and division. | This is necessary for growth and division. |
| 3. | It contains information about some properties of the cell. | It contains information about all the properties. |
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संबंधित प्रश्न
The biological scissor is ______.
Write a short note on Electrophoresis.
What are the restriction endonucleases? Give the rules of their nomenclature.
What is the cell that receives a recombinant gene called?
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Which of the following is an INCORRECT match?
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Name a recombinant vaccine that is currently being used in vaccination program?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Restriction enzymes were discovered by ______.
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
Importance of PCR.
Which one of the following enzymes is used to join DNA fragments?
Give a reason for the following:
DNA cannot enter directly into the host cell.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
 |
 |
 |
| Vector A | Vector B | Vector C |
