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प्रश्न
The biological scissor is ______.
पर्याय
restriction endonuclease
gyrase
DNA ligase
helicase
उत्तर
The biological scissor is restriction endonuclease.
APPEARS IN
संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
What does PCR stand for? Describe the different steps of PCR
The enzyme nuclease hydrolyses ______ of polynucleotide chain of DNA.
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
How gene amplification is done?
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
