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प्रश्न
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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| Vector A | Vector B | Vector C |
उत्तर
A cloning vector should have a replication origin that allows it to reproduce itself within the host cell. It should include a restriction site for the insertion of the target DNA. It should include a selectable marker with an antibiotic resistance gene to aid in the screening of the recombinant organism. By taking into account all of the above, She should select Vector-C.
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संबंधित प्रश्न
Give an example of the source of thermostable enzyme DNA polymerase.
Give an account of the Blue-White Method of selection of recombinants
What is gene cloning? Explain different tools used for it.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
The PCR technique is used for ______.
Plasmid is ______.
How gene amplification is done?
______technique is used for in vitro gene cloning or gene multiplication.
How many sets of primers are required in each cycle of PCR?
