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प्रश्न
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
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 |
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| Vector A | Vector B | Vector C |
उत्तर
A cloning vector should have a replication origin that allows it to reproduce itself within the host cell. It should include a restriction site for the insertion of the target DNA. It should include a selectable marker with an antibiotic resistance gene to aid in the screening of the recombinant organism. By taking into account all of the above, She should select Vector-C.
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संबंधित प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
What is PCR? Explain different steps involved in it.
From the following which one is NOT a feature of the plasmids?
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
The gene transfer between unrelated plants is brought about by ____________.
Exonuclease performs the function of ____________.
Which of these are most widely used in genetic engineering?
Plasmid is ______.
Name the enzyme responsible for the transcription of genetic code to produce mRNA.
